In 2017 TATAA Biocenter and the Institute of Biotechnology, BIOCEV, developed Two-tailed (2T) PCR for ultrasensitive analysis of microRNAs.
This highly specific, sensitive and cost-effective system is used to quantify miRNA expression based on RT-2T-qPCR, with SYBR-green or probe based detection. The technology also offers important specificity advantages for rare sequence detection using digital PCR.
HOW IT WORKS
microRNA analysis using
Two-Tailed PCR by professor Mikael
Microgenomics 2021 webinar EV & Circulating Biomarkers: M.Kubista
References & Papers
Two-Tailed PCR assays for microRNA
Quantification of microRNA editing with Two-Tailed PCR